The Sartorius Incucyte S3 Live Cell Imager is a sophisticated automated live-cell analysis system designed for continuous monitoring of biological processes within incubators. It offers high-resolution time-lapse imaging combined with environmental control, making it ideal for applications in cell biology, oncology, immunology, and drug discovery. The system integrates an imaging module and a control module for seamless operation. Its reliable construction and automated data acquisition allow researchers to study dynamic cellular events with minimal intervention. The Incucyte S3 enhances experimental reproducibility and throughput by providing real-time quantitative data without disturbing the cells.