The PerkinElmer IVIS Lumina S5 is an advanced in vivo imaging system designed for non-invasive optical imaging of live animals. It optimizes bioluminescence, fluorescence, and X-ray imaging modalities, enabling comprehensive preclinical research applications. The system features an integrated anesthesia unit for accurate physiological condition control during imaging procedures. Equipped with a cooled CCD camera and a configurable emission filter wheel, it provides high-sensitivity detection with adaptable imaging parameters. The Lumina S5’s ergonomic design and compact footprint facilitate seamless integration into laboratory environments. Its compatibility with the Living Image software suite simplifies image acquisition, analysis, and reporting, making it ideal for translational research and drug development studies. This system balances performance and ease of use to support reliable and reproducible in vivo imaging workflows.