The Caliper IVIS Lumina LT is an in vivo imaging system designed for high-sensitivity optical imaging of live small animals. It supports multi-modality imaging with fluorescence and bioluminescence capabilities. Featuring a sealed imaging chamber, it ensures reproducible imaging conditions with temperature and anesthesia control options via external accessories. The system is ideal for preclinical research in oncology, infectious disease, and gene expression studies. It includes comprehensive software for image acquisition and analysis, enabling quantification of biological processes in real time. Its robust design and workflow integration make it a reliable workhorse for laboratories requiring longitudinal animal imaging.